PGP9.5 Antibody

UCHL1/PGP9.5 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of PGP9.5 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is abundantly present in all neurons (accounts for 1-2% of total brain protein), expressed specifically in neurons and testis/ovary. [Wiki]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

UniProt

P09936

Host

Mouse

Reactivity

Human, Rat

Immunogen

Recombinant human protein was used as the immunogen for the PGP9.5 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

UBCE-L1

Applications

WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the PGP9.5 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This PGP9.5 antibody is available for research use only.

Storage Conditions

Store the PGP9.5 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic, ER membrane

Image Legend

IHC testing of FFPE rat cerebellum with PGP9.5 antibody (clone UBCE-L1) . Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.