Smooth Muscle Actin Antibody

Actins are a family of proteins which are highly conserved and play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. [RefSeq]

Product Specifications

CAS Number

9007-83-4

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

P62736

Host

Mouse

Reactivity

Human, Rat

Immunogen

Recombinant full-length human ACTA2 protein was used as the immunogen for the Smooth Muscle Actin antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

SMAT2-1

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the Smooth Muscle Actin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Smooth Muscle Actin antibody is available for research use only.

Storage Conditions

Store the Smooth Muscle Actin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human angiosarcoma with Smooth Muscle Actin antibody (clone SMAT2-1) . Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.