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Keratin 19 Antibody

Cytokeratin 19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Cytokeratin 19 is a type I keratin. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically found in the periderm, the transiently superficial layer that envelops the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [Wiki]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

UniProt

P08727

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human KRT19 protein was used as the immunogen for the Keratin 19 antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

CTKN19-1

Applications

IF, IHC-P, WB, FACS

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Keratin 19 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Keratin 19 antibody is available for research use only.

Storage Conditions

Store the Keratin 19 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic

Image Legend

Immunofluorescent staining of permeabilized human MCF7 cells with Cytokeratin 19 antibody (clone CTKN19-1, green) and Reddot nuclear stain (red) .

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