S100A9 Antibody

S100A9, also known as Calgranulin B or MRP14 (and Calprotectin L1H), is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis, adhesion, can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 and can induce degranulation of neutrophils by a MAPK-dependent mechanism. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT (1)

UniProt

P06702

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human protein was used as the immunogen for the S100A9 antibody.

Clonality

Monoclonal

Isotype

IgM k

Clone

PS1A9-1

Applications

IHC-P

Purity

PEG precipitation

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the S100A9 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This S100A9 antibody is available for research use only.

Storage Conditions

Store the S100A9 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Titering of the S100A9 antibody may be required for optimal performance.1. Not suitable for frozen tissue testing.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human tonsil with S100A9 antibody (clone PS1A9-1) . Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.