TRAcP Antibody / TRAP / ACP5

Tartrate-Resistant Acid Phosphatase is involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P13686

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human protein was used as the immunogen for the TRAcP antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

HACP5-1

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the TRAcP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This TRAcP antibody is available for research use only.

Storage Conditions

Store the TRAcP antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Titering of the TRAcP antibody may be required for optimal performance.

Location

Cytoplasmic (lysosomes)

Image Legend

IHC testing of FFPE human spleen with TRAcP antibody (clone HACP5-1) . FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.