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CD99 Antibody

CD99 is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, it may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of the protein. [Wiki]

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT (1), Prediluted IHC only format: incubate for 30 min at RT (2)

UniProt

P14209

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human protein was used as the immunogen for the CD99 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

CDLA99

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the CD99 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CD99 antibody is available for research use only.

Storage Conditions

Store the CD99 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cell surface

Image Legend

IHC testing of FFPE human Ewing's sarcoma and CD99 antibody (clone CDLA99) . Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

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