B2M Antibody

Beta-2 Microglobulin/B2M is a component of the class I major histocompatibility complex (MHC) . Involved in the presentation of peptide antigens to the immune system. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 2-4 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

UniProt

P61769

Host

Mouse

Reactivity

Human

Immunogen

Full length recombinant human protein was used as the immunogen for the B2M antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

MGBP2-1

Applications

IF, IHC-P, WB, FACS

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the B2M antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This B2M antibody is available for research use only.

Storage Conditions

Store the B2M antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human renal carcinoma with B2M antibody (clone MGBP2-1) . FFPE testing requires sections to be boiled inpH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.