Neurofilament Antibody (light chain)

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. [RefSeq]

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

UniProt

P07196

Host

Mouse

Reactivity

Human, Rat

Immunogen

Recombinant human neurofilament light chain protein was used as the immunogen for the Neurofilament antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

MSI27-1

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Neurofilament antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Neurofilament antibody is available for research use only.

Storage Conditions

Store the Neurofilament antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic, membranous

Image Legend

IHC testing of FFPE human cerebellum with Neurofilament antibody (clone MSI27-1) . Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.