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HSP60 Antibody / HSPD1

HSP60 has been implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

P10809

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human HSPD1 protein was used as the immunogen for the HSP60 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

SRPR60

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the HSP60 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This HSP60 antibody is available for research use only.

Storage Conditions

Store the HSP60 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Location

Cytoplasm (mitochondria)

Image Legend

IHC testing of FFPE human bladder carcinoma and HSP60 antibody (clone SRPR60) . Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

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