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HSP60 Antibody / HSPD1

HSP60 has been implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

UniProt

P10809

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human HSPD1 protein was used as the immunogen for the HSP60 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

SRPR60

Applications

IF, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the HSP60 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This HSP60 antibody is available for research use only.

Storage Conditions

Store the HSP60 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasm (mitochondria)

Image Legend

Immunofluorescent staining of MeOH-fixed human HeLa cells with HSP60 antibody (clone SRPR60, green) and Reddot nuclear stain (red) .

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