Cytokeratin 18 Antibody

Keratin 18 is a type I cytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. Keratin 18 is often used together with keratin 8 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood. [Wiki]

Product Specifications

CAS Number

9007-83-4

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

P05783

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human KRT18 protein was used as the immunogen for the Cytokeratin 18 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

CTKN18-1

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the Cytokeratin 18 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Cytokeratin 18 antibody is available for research use only.

Storage Conditions

Store the Cytokeratin 18 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human lung carcinoma and Cytokeratin 18 antibody (clone CTKN18-1) . FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 min, followed by cooling at RT for 20 min, prior to staining.