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Cytokeratin 18 Antibody

Keratin 18 is a type I cytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. Keratin 18 is often used together with keratin 8 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood. [Wiki]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

UniProt

P05783

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human KRT18 protein was used as the immunogen for the Cytokeratin 18 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

CTKN18-1

Applications

IF, IHC-P, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Cytokeratin 18 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Cytokeratin 18 antibody is available for research use only.

Storage Conditions

Store the Cytokeratin 18 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic

Image Legend

Immunofluorescent staining of permeabilized human MCF7 cells with Cytokeratin 18 antibody (clone CTKN18-1, green) and Reddot nuclear stain (red) .

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