Cytokeratin 7 Antibody

Cytokeratin-7 (CK7) is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. CK7 is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. It is found in simple glandular epithelia, and in transitional epithelium. Epithelial cells of the lung and breast both contain CK7, but some other glandular epithelia, such as those of the colon and prostate, do not. Because the protein is found in both healthy and neoplastic cells, antibodies to CK7 can be used in immunohistochemistry to distinguish ovarian and transitional cell carcinomas from colonic and prostate cancers, respectively. It is commonly used together with CK20 when making such diagnoses. [Wiki]

Product Specifications

CAS Number

9007-83-4

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

P08729

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human KRT7 protein was used as the immunogen for the Cytokeratin 7 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

CTKN7-1

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the Cytokeratin 7 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Cytokeratin 7 antibody is available for research use only.

Storage Conditions

Store the Cytokeratin 7 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human lung carcinoma and Cytokeratin 7 antibody (clone CTKN7-1) . Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.