Golgi Antibody / Golgi apparatus protein 1 / GLG1

Part of the cellular endomembrane system, the Golgi apparatus packages proteins into membrane-bound vesicles inside the cell before the vesicles are sent to their destination. The Golgi apparatus resides at the intersection of the secretory, lysosomal, and endocytic pathways. It is of particular importance in processing proteins for secretion, containing a set of glycosylation enzymes that attach various sugar monomers to proteins as the proteins move through the apparatus. [Wiki] <br>This antibody is useful in staining the Golgi apparatus in cell or tissue preparations and subcellular fractions.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

UniProt

Q92896

Host

Mouse

Reactivity

Human

Immunogen

The Golgi fraction from human liver cells was used as the immunogen for the Golgi antibody. Not suitable for mouse and rat sample testing.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

CRFG1

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Golgi antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Golgi antibody is available for research use only.

Storage Conditions

Store the Golgi antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Golgi complex in cytoplasm

Image Legend

IHC testing of FFPE human pancreas and Golgi antibody (clone CRFG1) . FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA followed by cooling at RT for 20 min., prior to staining.