Prolactin Receptor Antibody

Prolactin is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. The initial step in its action is the binding to a specific membrane receptor, which belongs to the superfamily of class 1 cytokine receptors. The function of the prolactin receptor is mediated, at least in part, by two families of signaling molecules: Janus kinases and signal transducers and activators of transcription.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human Prolactin receptor was used as the immunogen for this antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

ERSV8-1

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the Prolactin receptor antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Prolactin receptor antibody is available for research use only.

Storage Conditions

Store the Prolactin receptor antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Prolactin receptor antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Location

Cell surface and cytoplasmic

Image Legend

IHC analysis of Prolactin receptor antibody (clone ERSV8-1) and testicular carcinoma. Required HIER: digestion of tissue sections with pepsin at 1 mg/mL in Tris-HCl, pH 2, for 15 min at RT or 10 min at 37oC.