SUMO1 Antibody

The small ubiquitin-related modifier (SUMO) proteins, which include SUMO1, 2 and 3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

Host

Mouse

Reactivity

Human, Rat

Immunogen

Recombinant human protein was used as the immunogen for this SUMO1 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

S1MT-2

Applications

WB, IHC-P, IF

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the SUMO1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This SUMO1 antibody is available for research use only.

Storage Conditions

Store the SUMO1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Titering of the SUMO1 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.