FOXP3 Antibody

The FOX family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. FOXP3 is essential for normal immune homeostasis. It represses transcription through a DNA binding forkhead domain, thereby regulating T cell activation.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

Host

Mouse

Reactivity

Human

Immunogen

An N-terminal recombinant protein fragment was used as the immunogen for this FOXP3 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

FHBP3-1

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the FOXP3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This FOXP3 antibody is available for research use only.

Storage Conditions

Store the FOXP3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Titering of the FOXP3 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Location

Predominantly nuclear, some cytoplasmic

Image Legend

IHC analysis of FOXP3 antibody (clone FHBP3-1) and human tonsil tissue. Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min.