DOG1 Antibody

Expression of TMEM16A (DOG1) protein is elevated in the gastrointestinal stromal tumors (GIST's), c-kit signaling-driven mesenchymal tumors of the GI tract. It is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for DOG11 has been reported in 97.8 percent of scorable GIST's, including all c-kit negative GIST's. Overexpression has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha (PDGFR alpha) mutants that fail to express c-kit antigen. The overall sensitivity of TMEM16A and c-kit in GIST's is nearly identical: 94.4% vs. 94.7%.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human protein was used as the immunogen for this DOG1 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

TDAN4

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the DOG1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This DOG1 antibody is available for research use only.

Storage Conditions

Store the DOG1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Titering of the DOG1 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Location

Cell Surface and Cytoplasmic

Image Legend

IHC testing of human gastrointestinal stromal tumor (GIST) and DOG1 antibody (clone tDAN4) . Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.