Calponin Antibody
Calponin is a calmodulin, F-actin and tropomyosin binding protein which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)
UniProt
P51911
Host
Mouse
Reactivity
Human, Rat
Immunogen
Recombinant human protein was used as the immunogen for the Calponin antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
CLPN5-1
Applications
IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Calponin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Calponin antibody is available for research use only.
Storage Conditions
Store the Calponin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Location
Cytoplasmic
Image Legend
Immunofluorescent staining of PFA-fixed human K562 cells with Calponin antibody (green, clone CLPN5-1) and Reddot nuclear stain (red) .
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