P27Kip1 Antibody

This mAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow Cytometry: 1-2ug/million cells in 0.1ml, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

UniProt

P46527

Host

Mouse

Reactivity

Human, Mouse and Rat

Immunogen

Recombinant human protein was used as the immunogen for the p27Kip1 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

KIP27-1

Applications

FACS, IF, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the p27Kip1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This p27Kip1 antibody is available for research use only.

Storage Conditions

Store the p27Kip1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Nuclear

Image Legend

IHC testing of FFPE human prostate cancer and p27Kip1 antibody (clone KIP27-1) . Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.