Cyclin D1 Antibody / PE Conjugate

This antibody recognizes a protein of 36kDa, identified as Cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t (11; 14) (q13; q32) translocation resulting in over-expression. The antibody is useful in identifying mantle cell lymphomas (Cyclin D1 positive) from CLL/SLL and follicular lymphomas (Cyclin D1 negative) . Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow Cytometry: 5ul/test/million cells or 5ul/test/100ul of whole blood

Host

Mouse

Reactivity

Human

Immunogen

Human recombinant full length Cyclin D1 protein was used as the immunogen.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

BCLD1-1

Applications

FACS

Purity

Protein G affinity chromatography

Format

PE Conjugate

Buffer

1X PBS, pH 7.4

Reconstitution

Store the Cyclin D1 antibody at 2-8oC. Conjugate is light sensitive, store in the dark.

Limitations

This Cyclin D1 antibody is available for research use only.

Storage Conditions

Store the Cyclin D1 antibody at 2-8°C. Conjugate is light sensitive, store in the dark.

Formulation

500 ul at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

The dilution stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cyclin D1 antibody to be titered up or down for optimal performance.