EGFR Antibody
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. [UniProt]
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/million cells in 0.1ml, Immunofluorescence: 1-2 µg/mL
UniProt
P00533
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for this EGFR antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
ERB59-1
Applications
FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the EGFR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This EGFR antibody is available for research use only.
Storage Conditions
Store the EGFR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Location
Cell surface
Image Legend
Immunofluorescent testing of A431 cells and Alexa Fluor conjugated EGFR antibody (green, clone ERB59-1) with DAPI nuclear stain (blue) .
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