Anti-IgM Antibody
IgM is the first antibody generated in an immune response to an antigen. It is generally a pentamer with each of the five immunoglobulins linked together with disulfide bonds. In its pentamer form, it has a molecular mass of 970 kDa and 10 antigen binding sites (due to the large size of most antigens, not all binding sites can be filled simultaneously. IgM antibodies account for approximately 5%-10% of all the antibody in the body.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)
Host
Mouse
Reactivity
Human
Immunogen
Human Mu heavy chain was used as the immunogen for this IgM antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
MuHC2
Applications
WB, IHC-P
Purity
Protein G
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the IgM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This IgM antibody is available for research use only.
Storage Conditions
Store the IgM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Product Datasheet
https://www.nsjbio.com/tds-pdf/anti-igm-antibody-muhc2-v7033
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Location
Cytoplasm, Cell Surface and Secreted
Image Legend
Western blot analysis of IgM antibody and Raji cell lysate (clone MuHC2) . Expected molecular weight: 70-75 kDa.
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