Anti-IgM Antibody

IgM is the first antibody generated in an immune response to an antigen. It is generally a pentamer with each of the five immunoglobulins linked together with disulfide bonds. In its pentamer form, it has a molecular mass of 970 kDa and 10 antigen binding sites (due to the large size of most antigens, not all binding sites can be filled simultaneously. IgM antibodies account for approximately 5%-10% of all the antibody in the body.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

Host

Mouse

Reactivity

Human

Immunogen

Human Mu heavy chain was used as the immunogen for this IgM antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

MuHC2

Applications

WB, IHC-P

Purity

Protein G

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the IgM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This IgM antibody is available for research use only.

Storage Conditions

Store the IgM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Due to differences in protocols and secondaries, the IgM antibody may need to be titered for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.