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Glypican-3 Antibody

Glypican-3 is a proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT (1), Prediluted IHC only format: incubate for 30 min at RT (2)

UniProt

P51654

Host

Mouse

Reactivity

Human, Rat

Immunogen

Recombinant full-length human GPC3 protein was used as the immunogen for the Glypican-3 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

SGPN3-1

Applications

IF, IHC-P, FACS

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Glypican-3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Glypican-3 antibody is available for research use only.

Storage Conditions

Store the Glypican-3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic

Image Legend

Immunofluorescent staining of methanol-fixed HepG2 cells with Glypican-3 antibody (green, clone SGPN3-1) and Reddot nuclear stain (red) .

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