HCG alpha Antibody / Luteinizing Hormone alpha

This antibody reacts with a protein of ~13kDa, identified as alpha sub-unit of hCG. HCG is a glycoprotein which is secreted in large quantities by normal trophoblasts. HCG is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH) .

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

UniProt

P01215

Host

Mouse

Reactivity

Human

Immunogen

Recombinant protein was used as the immunogen for this hCG alpha antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

CGA91-2

Applications

IHC-P

Purity

Protein G purified monoclonal antibody

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the hCG alpha antibody at 2-8oC.

Limitations

This hCG alpha antibody is available for research use only.

Storage Conditions

Store the hCG alpha antibody at 2-8°C.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Variations in protocols, secondaries and substrates may require the hCG alpha antibody to be titered for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.