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EpCAM Antibody

EpCAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/million cells in 0.1ml, Western blot: 2-4 µg/mL, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT (1), Prediluted IHC only format: incubate for 30 min at RT (2)

UniProt

P16422

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human protein was used as the immunogen for the EpCAM antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

EPM17-1

Applications

WB, IHC-P, FACS, IF

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This EpCAM antibody is available for research use only.

Storage Conditions

Store the EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cell surface, cytoplasmic

Image Legend

Immunofluorescent staining of human MCF7 cells with EpCAM antibody (clone EPM17-1, green) and Reddot nuclear stain (red) .

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