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Vimentin Antibody

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. It is involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

P08670

Host

Mouse

Reactivity

Human

Immunogen

Full length human recombinant protein was used as the immunogen for the Vimentin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

VIM66-1

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the Vimentin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Vimentin antibody is available for research use only.

Storage Conditions

Store the Vimentin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Location

Cytoplasmic

Image Legend

IHC analysis of FFPE human melanoma stained with Vimentin antibody (clone VIM66-1) .

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