Vimentin Antibody
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. It is involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. [UniProt]
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT (1), Prediluted IHC only format: incubate for 30 min at RT (2)
UniProt
P08670
Host
Mouse
Reactivity
Human
Immunogen
Full length human recombinant protein was used as the immunogen for the Vimentin antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
VIM66-1
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the Vimentin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Vimentin antibody is available for research use only.
Storage Conditions
Store the Vimentin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic
Image Legend
Western blot testing of Vimentin antibody (clone VIM66-1) and Raji cell lysate. Predicted molecular weight ~58 kDa.
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