14-3-3 Sigma Antibody

SFN (Stratifin), also called 14-3-3 Sigma, is a p53-induced tumor suppressor gene which is activated in response to DNA damage, causing cell cycle arrest at G2 phase by blocking cdc2-cyclin B1 complex from entering the nucleus. It is inactivated in breast, lung, prostate, liver and gastric cancer. It is associated with poor prognosis when its down-regulation is observed in epithelial ovarian cancer. SFN expression could contribute to cancer cell proliferation and the development and/or progression of human gastrointestinal cancer.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

UniProt

P31947

Host

Mouse

Reactivity

Human

Immunogen

Full length recombinant human protein was used as the immunogen for the 14-3-3 Sigma antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

CPTC-SFN-2

Applications

WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the 14-3-3 Sigma antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This 14-3-3 Sigma antibody is available for research use only.

Storage Conditions

Store the 14-3-3 Sigma antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Location

Cytoplasmic, nuclear

Image Legend

IHC staining of FFPE human skin with 14-3-3 Sigma antibody (clone CPTC-SFN-2) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.