Recombinant Placental Alkaline Phosphatase Antibody
PLAP is a tissue specific, trophoblast-derived, 70kDa, glycosyl-phosphatidylinositol (GPI) -anchored, dimeric, Zn2+ metallo-glycoprotein that catalyzes the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. It is present in the placenta and serum of pregnant women and in high frequency in gynecological and testicular cancers and in lower frequency in other tumors. The three tissue-specific AP's in humans, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98% homologous. Non-tissue specific AP is found in kidney, liver and bone. This mAb binds equally well to all common allelic variants (S, F, FS and I) of PLAP and to some variants of AP from normal human testis. This mAb can be used as tracer antibody in ELISA to detect PLAP in serum of S, F, FS and I phenotypes.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL for 30 min at RT, Western blot: 1-2 µg/mL
UniProt
P05187
Host
Rabbit
Reactivity
Human
Immunogen
Recombinant full-length human protein was used as the immunogen for the recombinant Placental Alkaline Phosphatase antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
ALPP/2899R
Type
Recombinant
Applications
IHC-P, WB
Purity
Protein A affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the recombinant Placental Alkaline Phosphatase antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant Placental Alkaline Phosphatase antibody is available for research use only.
Storage Conditions
Store the recombinant Placental Alkaline Phosphatase antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic and cell surface
Image Legend
IHC testing of FFPE human placenta with recombinant Placental Alkaline Phosphatase antibody (clone ALPP/2899R) .
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