What is the catalog number for CD5 Antibody?

The catalog number for CD5 Antibody is V3961-20UG.

What sizes are available for CD5 Antibody?

CD5 Antibody is available in the following size(s): 20 µg.

Is CD5 Antibody in stock?

CD5 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

CD5 Antibody

Recognizes a 67kDa transmembrane protein which is identified as CD5. The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-) . Anti-CD5 does not react with granulocytes or monocytes.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/10^6 cells, Western blot: 1-2 µg/mL, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P06127

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 269-366 from the human protein was used as the immunogen for this CD5 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

CD5/2416

Applications

FACS, WB, IF, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the CD5 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CD5 antibody is available for research use only.

Storage Conditions

Store the CD5 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The optimal dilution of the CD5 antibody for each application should be determined by the researcher.

Location

Cell surface

Image Legend

IHC staining of FFPE human tonsil with CD5 antibody (clone CD5/2416) . Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.