Vinculin Antibody
Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and alpha-Actinin. In addition, vinculin, Talin and alpha-Actinin each contain Actin binding sites. Expression of vinculin and Talin have been shown to be affected by the level of Actin expression. Alpha-Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P18206
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 174-322 from the human protein was used as the immunogen for this Vinculin antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
VCL/2575
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Vinculin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Vinculin antibody is available for research use only.
Storage Conditions
Store the Vinculin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Vinculin antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Western blot testing of 1) human A431, 2) human K562 and 3) mouse NIH 3T3 cell lysate with Vinculin antibody. Predicted molecular weight ~124 kDa.
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