ZAP70 Antibody
ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.
Product Specifications
CAS Number
9007-83-4
Specifications
UniProt
P43403
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 247-382 from the human protein was used as the immunogen for the ZAP70 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
ZAP70/2047
Applications
ELISA, FACS, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the ZAP70 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This ZAP70 antibody is available for research use only.
Storage Conditions
Store the ZAP70 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the ZAP70 antibody should be determined by the researcher.
Location
Cytoplasmic, cell surface
Image Legend
Flow testing of PFA-fixed Jurkat cells with ZAP70 antibody (clone ZAP70/2047) ; Red=isotype control, Blue= ZAP70 antibody.
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