ZAP70 Antibody

ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, ELISA: 2-4 µg/mL (order BSA/azide-free format), Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P43403

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 247-382 from the human protein was used as the immunogen for the ZAP70 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

ZAP70/2035

Applications

FACS, IF, ELISA, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the ZAP70 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This ZAP70 antibody is available for research use only.

Storage Conditions

Store the ZAP70 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the ZAP70 antibody should be determined by the researcher.

Location

Cytoplasmic, cell surface

Image Legend

IHC testing of FFPE human tonsil tissue with ZAP70 antibody (clone ZAP70/2035) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.