Ubiquitin Antibody

Ubiquitin is a highly conserved and plays an essential role in the ubiquitin-proteasome pathway. In ubiquitination process, it is first activated by forming a thiol-ester complex with the activation component E1, which is then transferred to ubiquitin-carrier protein E2, followed by to ubiquitin ligase E3 for final delivery to epsilon-NH2 of the target protein lysine residue. IkB, p53, cdc25A, Bcl-2 etc. are shown as targets of ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis. Moreover, ubiquitin have been reported to bind covalently with pathological inclusions which are resistant to degradation e.g. neurofibrillary tangles/paired helical filaments in Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease etc.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order BSA/sodium azide-free format for coating), Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P0CG47, P0CG48, P62979, P62987, P62988

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 1-119 from the human protein was used as the immunogen for the Ubiquitin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

UBB/2122

Applications

ELISA, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Ubiquitin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Ubiquitin antibody is available for research use only.

Storage Conditions

Store the Ubiquitin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Ubiquitin antibody should be determined by the researcher.

Location

Cell Surface, cytoplasmic, nuclear

Image Legend

IHC staining of FFPE human brain with Ubiquitin antibody (clone UBB/2122) . Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.