Thyroid Peroxidase Antibody

Thyroperoxidase (TPO) is a 933-amino acid, type I transmembrane glycoprotein that plays a key role in thyroid gland function and autoimmunity. It is present as a dimer on the apical surface of thyroid follicular cells. TPO functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Malignant thyroid tumors exhibit an anomaly in TPO resulting in lower affinity for anti-TPO. This antibody may aid in the differentiation between benign and malignant thyroid tumors.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P07202

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 685-804 from the human protein was used as the immunogen for the Thyroid Peroxidase antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

TPO/1922

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Thyroid Peroxidase antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Thyroid Peroxidase antibody is available for research use only.

Storage Conditions

Store the Thyroid Peroxidase antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Thyroid Peroxidase antibody should be determined by the researcher.

Location

Cytoplasmic, cell surface

Image Legend

IHC testing of FFPE human thyroid carcinoma stained with Thyroid Peroxidase antibody (clone TPO/1922) . Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.