Melanoma gp100 Antibody / PMEL17

The gp100 molecule is a 100kDa glycosylated protein that is cleaved into a small (26kDa) carboxy-terminal fragment and a larger amino- terminal section (60-64 kDa), which is subsequently cleaved to generate 26kDa and 34-38kDa fragments.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: 2-4 µg/mL (order BSA/azide-free format), Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 1-2 µg/mL

UniProt

P40967

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 376-502 from the human protein was used as the immunogen for the Melanoma gp100 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

PMEL/2038

Applications

ELISA, IHC-P, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Melanoma gp100 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Melanoma gp100 antibody is available for research use only.

Storage Conditions

Store the Melanoma gp100 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Melanoma gp100 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC testing of human melanoma with Melanoma gp100 antibody (clone PMEL/2038) . Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.