PMEL17 Antibody / Melanoma gp100

The gp100 molecule is a 100kDa glycosylated protein that is cleaved into a small (26kDa) carboxy-terminal fragment and a larger amino- terminal section (60-64 kDa), which is subsequently cleaved to generate 26kDa and 34-38kDa fragments.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: 2-4 µg/mL (order BSA/azide-free format), Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 1-2 µg/mL

UniProt

P40967

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 376-502 from the human protein was used as the immunogen for the PMEL17 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

PMEL/2037

Applications

ELISA, IHC-P, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the PMEL17 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This PMEL17 antibody is available for research use only.

Storage Conditions

Store the PMEL17 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the PMEL17 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC testing of human melanoma with PMEL17 antibody (clone PMEL/2037) . Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.