BrdU Antibody / Bromodeoxyuridine

This antibody reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to Bromodeoxyuridine / BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

Host

Rabbit

Reactivity

All Species Expected

Immunogen

BrdU / Bromodeoxyuridine conjugated to KLH was used as the immunogen for this BrdU antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

BRD2888R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the BrdU antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This BrdU antibody is available for research use only.

Storage Conditions

Store the BrdU antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Nuclear

Image Legend

IHC staining of BrdU-incorporated FFPE mouse kidney with BrdU antibody (clone BRD2888R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.