TLE1 Antibody / Transducin-like enhancer protein 1
Key players in the Notch pathway are the TLE genes, which are human homologs of the Drosophila groucho gene. Groucho is a transcriptional repressor that plays a key role in neurogenesis, segmentation and sex determination. Transducin-like enhancer protein 1 (TLE1) is a protein that is encoded by the TLE1 gene and is involved in control of hematopoiesis, neuronal, and terminal epithelial differentiation. Positive immunohistochemical nuclear staining with anti-TLE-1 has been shown to be a useful addition to an IHC panel when differentiating synovial sarcoma from other soft tissue malignancies.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
Q04724
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 175-338 from the human protein was used as the immunogen for the TLE1 antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
TLE1/2085
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the TLE1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This TLE1 antibody is available for research use only.
Storage Conditions
Store the TLE1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the TLE1 antibody should be determined by the researcher.
Location
Nuclear
Image Legend
Western blot testing of human HeLa cell lysate with TLE1 antibody (clone TLE1/2085) . Predicted molecular weight ~83 kDa.
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