TLE1 Antibody / Transducin-like enhancer protein 1

Key players in the Notch pathway are the TLE genes, which are human homologs of the Drosophila groucho gene. Groucho is a transcriptional repressor that plays a key role in neurogenesis, segmentation and sex determination. Transducin-like enhancer protein 1 (TLE1) is a protein that is encoded by the TLE1 gene and is involved in control of hematopoiesis, neuronal, and terminal epithelial differentiation. Positive immunohistochemical nuclear staining with anti-TLE-1 has been shown to be a useful addition to an IHC panel when differentiating synovial sarcoma from other soft tissue malignancies.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q04724

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 175-338 from the human protein was used as the immunogen for the TLE1 antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

TLE1/2051

Applications

WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the TLE1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This TLE1 antibody is available for research use only.

Storage Conditions

Store the TLE1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the TLE1 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC testing of FFPE human endometrial carcinoma with TLE1 antibody (clone TLE1/2051) . Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.