PTEN Antibody

PTEN (phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase) contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. It was identified as a tumor suppressor that is mutated in a large number of cancers, including sporadic brain, breast, kidney, and prostate cancers.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P60484

Host

Mouse

Reactivity

Human

Immunogen

Full length human recombinant protein was used as the immunogen for this PTEN antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

PTEN/2110

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the PTEN antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This PTEN antibody is available for research use only.

Storage Conditions

Store the PTEN antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the PTEN antibody should be determined by the researcher.

Location

Cytoplasmic, nuclear

Image Legend

IHC testing of human prostate carcinoma with PTEN antibody (clone PTEN/2110) . Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.