Podocalyxin Antibody

Podocalyxin/PODXL is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

O00592

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 310-447 from the human protein was used as the immunogen for the Podocalyxin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

PODXL/2185

Applications

IHC-P

Purity

Protein G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Podocalyxin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Podocalyxin antibody is available for research use only.

Storage Conditions

Store the Podocalyxin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Podocalyxin antibody should be determined by the researcher.

Location

Cell surface and cytoplasmic

Image Legend

IHC testing of FFPE human endometrium with Podocalyxin antibody (clone PODXL/2185) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.