Melanoma gp100 Antibody / PMEL17

PMEL17/Melanoma gp100/SILV plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P40967

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 376-502 from the human protein was used as the immunogen for the Melanoma gp100 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

MSSG95-2

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the Melanoma gp100 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Melanoma gp100 antibody is available for research use only.

Storage Conditions

Store the Melanoma gp100 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Melanoma gp100 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of human melanoma with Melanoma gp100 antibody (clone MSSG95-2) . Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.