Nucleophosmin Antibody
Recognizes a 33kDa glycoprotein, identified as Nucleophosmin (NPM) . It is predominantly localized in the nucleus of cells in most tissues. NPM is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. This phosphoprotein moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location. Mutations in this gene are associated with acute myeloid leukemia. The antibody may be a useful aid for classification of acute myeloid leukemia.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL
UniProt
P06748
Host
Mouse
Reactivity
Human
Immunogen
Clonality
Monoclonal
Isotype
IgG1 k
Clone
NA24
Applications
IHC-P, IF, FACS
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Nucleophosmin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Nucleophosmin antibody is available for research use only.
Storage Conditions
Store the Nucleophosmin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Nucleophosmin antibody should be determined by the researcher.
Location
Nuclear, cytoplasmic
Image Legend
Immunofluorescent staining of human HeLa cells with Nucleophosmin antibody (clone NA24, green) and phalloidin (red) .
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