BOB1 Antibody

BOB1, also called POU2AF1, expression in a variety of established B-cell lines, representing different stages of B-cell development, has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immuno-positive for BOB1. Conversely, only some cases of classical Hodgkin lymphoma show BOB1 immuno-reactivity within the Hodgkin and Reed-Sternberg cells. Expression of BOB1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of acute myeloid leukemia. B-CLL, marginal zone lymphoma, and mantle cell lymphoma may show weak to moderate immunoreactivity.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q16633

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 148-255 from the human protein was used as the immunogen for the BOB1 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

BOB1/2425

Applications

WB, IHC-P

Purity

Protein G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the BOB1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This BOB1 antibody is available for research use only.

Storage Conditions

Store the BOB1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the BOB1 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

Western blot testing of human Raji cell lysate with BOB1 antibody (clone BOB1/2425) . Predicted molecular weight: ~28 kDa (unmodified), 35-40 kDa (ubiquitinated) .