BOB-1 Antibody
BOB-1, also called POU2AF1, expression in a variety of established B-cell lines, representing different stages of B-cell development, has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immuno-positive for BOB-1. Conversely, only some cases of classical Hodgkin lymphoma show BOB-1 immuno-reactivity within the Hodgkin and Reed-Sternberg cells. Expression of BOB-1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of acute myeloid leukemia. B-CLL, marginal zone lymphoma, and mantle cell lymphoma may show weak to moderate immunoreactivity.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2 µg/mL, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
Q16633
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 148-255 from the human protein was used as the immunogen for the BOB-1 antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
BOB1/2424
Applications
FACS, IF, WB, IHC-P
Purity
Protein G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the BOB-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This BOB-1 antibody is available for research use only.
Storage Conditions
Store the BOB-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the BOB-1 antibody should be determined by the researcher.
Location
Nuclear
Image Legend
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