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OCT-2 Antibody / POU2F2

Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3') . Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P09086

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 112-297 from the human protein was used as the immunogen for the OCT-2 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

PD2F2-1

Applications

IHC-P, WB

Purity

Protein G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the OCT-2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This OCT-2 antibody is available for research use only.

Storage Conditions

Store the OCT-2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the OCT-2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear

Image Legend

IHC testing of FFPE human tonsil with OCT-2 antibody (clone PD2F2-1) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

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