TIMP2 Antibody

It recognizes a protein of 21kDa, identified as tissue inhibitor of metalloproteinases 2 (TIMP2) . It is closely related to TIMP1 and shows the highest binding affinity to both the latent (pro) and active forms of 72kDa Type IV collagenase (also known as MMP2 or gelatinase A) . It also has affinity for the active form of 92kDa Type IV collagenase (also known as MMP9 or gelatinase B) . TIMP2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: 2-4 µg/mL (order BSA/azide-free format), Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P16035

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 48-220 from the human protein was used as the immunogen for this TIMP2 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

TIMP2/2044

Applications

ELISA, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the TIMP2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This TIMP2 antibody is available for research use only.

Storage Conditions

Store the TIMP2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TIMP2 antibody to be titered up or down for optimal performance.

Location

Cytoplasmic, cell surface, secreted

Image Legend

IHC testing of FFPE human placenta with TIMP2 antibody (clone TIMP2/2044) . Required HIER: requires boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.