StAR Antibody / Steroidogenic acute regulatory protein

Steroidogenic Acute Regulatory Protein (STAR) controls the rate-limiting step of steroidegenesis by translocating cholesterol from the outer mitochondrial membrane to the inner membrane where it is later cleaved to pregnenolone. It is primarily present in steroid-producing cells, including Leydig cells in the testis, theca cells and luteal cells in the ovary and adrenal cells in the adrenal cortex. Due to low levels of pregnenolone, seminomas and Leydig cell tumors display no specific STAR staining. Therefore, STAR antibody may assist in differentiating sex cord stromal tumors (SCST), seminomas and embryonal carcinomas.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P49675

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 39-108 from the human protein was used as the immunogen for this StAR antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

STAR/2140

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the StAR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This StAR antibody is available for research use only.

Storage Conditions

Store the StAR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the StAR antibody to be titered up or down for optimal performance.

Location

Cytoplasmic (mitochondrial)

Image Legend

IHC staining of FFPE human adrenal gland with StAR antibody (clone STAR/2140) . Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.